Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Bovine T-cell Surface Glycoprotein CD8 Alpha Chain (also known as CD8A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine CD8A antibody. CD8A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CD8A Antibody is added and binds to CD8A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD8A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine CD8A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm